TWO DELIVERIES / FIVE PASSAGES
RETROSPECTIVE / 8 PAIRED RUNSCompare the same stanza on each recording’s own clock. Sound and neural activity retain their original timing. These level-matched recordings use the original frozen fly simulator.
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Switching recordings pauses playback and goes to the corresponding stanza’s beginning. Human timestamps are approximate; highlighting is a guide. Playback stops at the selected stanza’s end.
VOICE
RESPONSE / direct auditory downstream cells
Green: recorded mean response minus matched silence, Hz/neuron. Band: range of eight runs. Dashed rust: input-only prediction. Complete 100 ms bins; no time stretching.
INJECTED INPUT
All five stanza comparisons
Human minus synthetic downstream mean rate, each relative to matched silence. “Robust” requires the declared directional check to survive all nine shifts of the approximate human boundaries (±0.2 s). This is descriptive, not statistical significance.
| Stanza | Δ Hz/neuron | Runs higher / lower | Boundary robust |
|---|
READING / interpretation of the comparison
First recording: synthetic reference. Second recording: Denny Sayers. This reading concerns pooled downstream activity; population-specific results follow below.
WHICH POPULATIONS DIFFER?
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Buttons navigate to the corresponding audio stanza; the charts above continue to show pooled downstream activity. Human minus synthetic, each relative to silence. Candidates were selected on earlier runs, then checked on different noise seeds without refitting or reselection. “Survives” requires raw and residual differences to retain their direction under all three amplitude approximations and all nine boundary shifts.
| Type / stanza | Cells total / direct | Held-out raw Δ Hz/neuron | Residual Δ range* | Survives? |
|---|
*Range across the three baseline-subtracted means at the nominal boundaries; all boundary checks are in the evidence JSON. Whole annotated types are measured, including cells outside the direct input targets. These small populations have five to seven cells; their rates do not represent the whole fly. No behavioral function is assigned from their names.
READING / population-specific response
Population screen: scope and evidence
This is a bounded retrospective screen of 70 types with at least five cells and three directly connected auditory targets, excluding injected cells, blank annotations and composite labels. 350 type/stanza pairs were screened. The gates are descriptive, not significance tests or correction for multiple comparisons. Validation repeats the same performances with new simulator noise; it does not validate across new speakers or biological flies. Residuals test three simple approximations, not all amplitude-based explanations.
Small named motor circuits fall outside this eligibility rule. The surviving labels do not establish a feeling, percept or disposition toward a specific action. No simulator weights or dynamics changed.
Method, limits and measured evidence
The comparators are external regressions fitted on earlier synthetic runs (seeds 64–71). The fly is never trained or changed. Evaluation uses seeds 101–108; the human performance is not used for fitting. Reference evaluation repeats the calibration stimulus with new noise. All three comparators are retained. Residuals are approximation errors, not proof of a special connectome contribution.
Equal RMS does not mean equal stimulation. Human audio lasts 45.1 s versus 26.17 s and has 10 capped input frames. Stanza means exclude inter-stanza gaps. Human alignment is approximate; the boundary check does not validate it. Performance, duration and acoustics differ together, so this is not a causal effect of reader identity. The interpreter receives numerical response summaries, never the poem.
WHERE EMPHASIS FALLS
RECORDED ENSEMBLE / 8 PAIRED RUNSThe same voice and timing. Energy moves between two passages; every other audio sample stays unchanged. These are smoothly edited amplitude contrasts, not new performances or changes in intonation.
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VOICE
READYRESPONSE / direct auditory downstream cells
Mean firing relative to matched silence, Hz/neuron, in 100 ms bins. Bands are the observed range of eight runs, not confidence intervals. The cursor follows audio playback; this is a recorded ensemble, not one live fly.
What changed at the altered passages?
READING / interpretation of this comparison
Method and measured evidence
Targets use source-line intervals 2 and 17. The gain envelope transfers equal waveform energy between them, with 80 ms tapered edges. RMS is matched within PCM rounding; total injected drive is measured separately. No compression, pitch, timing or connectome changes. The amplitude edit has not been independently reviewed for naturalness.
Local windows and the response criterion were declared before 32 simulations with seeds 301–308. Criterion: temporal mean difference RMS exceeds across-seed variability RMS and the two four-run halves have cosine similarity above 0.5. This is a descriptive check, not statistical significance. Off-target means exclude both target windows and their 0.3-second extensions. The reading receives only response measurements, never poem text. No movement, feeling or preference is established.
Fixed English voice. Up to 2,000 characters / 60 seconds of audio.
The voice is standardized.
The nervous system remains frozen.
LOADING CONNECTOME
The response is computed before synchronized playback.
01 / VOICE
READYBody: NeuroMechFly micro-CT anatomy. Static pose; no simulated movement.
Rings: audio amplitude. Antennal color: recorded JON firing.
Press play to hear the poem and replay its recorded neural trajectory.
02 / NEURAL RESPONSE
00:00.00Population values: current recorded 20 ms rates. The accompanying difference is the full-duration mean against matched silence; these groups can overlap.
POEM / MATCHED SILENCE
SAME SEED · SAME DURATION · ZERO CONTROL INPUTOne matched pair. This separates sound-driven changes from ongoing model activity for this seed; it does not establish a typical biological response.
READING
INTERPRETATION OF THE RESPONSEWhat this could mean for the fly
Known circuit functions help interpret the response. They do not turn simulated spikes into a report of subjective experience.
Composed by transparent rules from the measurements alone. The interpreter received no poem text.
Exactly what the interpreter received
RESPONSE
SIMULATOR-DERIVED MEASUREMENTSWhat happened in the simulated nervous system.
Largest population changes
Mean Hz/neuron during audio. For controlled records, Δ compares the matched silence run.
| Cell type | n | During | Δ reference |
|---|
Monitored pathways & outputs
Anatomical groups and identified cell types; no inferred behavior.
| Population | n | During | Δ reference |
|---|
Activity rankings & temporal events
Highest mean activity
| Cell type | n | During | Δ reference |
|---|
Matched records rank changes against equal-duration silence. The opening baseline remains a separate descriptive measure. One paired seed is not a significance test; small populations can dominate rankings.
PROVENANCE / inspect & download
METHOD A voice, an auditory input, a frozen connectome.
How the encounter unfolds: temporal confirmation ↗
Auditory receiver laboratory: development and validation ↗
Does earlier sound change reception of an identical passage? ↗
Delivery bench: compare level-matched performances across repeated seeds ↗
The reference poem is rendered by one fixed synthetic voice. A separate, credited human performance is available for comparison; each recording receives its own simulation and matched silence control. No semantic representation is provided to the fly. Frame-by-frame sound amplitude becomes equal stimulation of 138 annotated JO-A/JO-B Johnston’s-organ neurons.
The full fly.ai connectome simulation runs with its weights frozen and its seeded noise intact. RESPONSE comes from recorded simulated spikes. READING interprets a restricted measurement summary and never sees the poem.
The 3D body uses NeuroMechFly anatomy from a female micro-CT exemplar; it is an illustrative surface, separate from the MaleCNS connectome. The static pose does not simulate listening mechanics or behavior. Sound rings display amplitude; antennal color displays JON firing. Anatomy credits ↗
The acoustic bridge is deliberately crude: it measures RMS amplitude, without modeling frequency tuning or antennal mechanics. This is a computational artwork and critical apparatus, not evidence that Drosophila understands poetry.
Critical directions: affect, performance, and posthumanities ↗
Read the full method and modeling choices ↗